Fat Analysis & Fatty Acid Profile Testing
Know what's in your lipid fraction
Accurate fat and fatty acid testing is essential for nutrition labeling, product development, quality control, and ingredient verification. At Twin Arbor Analytical, we provide comprehensive lipid analysis for foods, dietary supplements, oils, softgels, powders, beverages, and functional ingredients.
Our laboratory offers total fat analysis by CEM ORACLE, fatty acid methyl ester (FAMEs) profiling by GC-FID, cholesterol analysis by derivatization with GC-FID, and peroxide value testing — delivering reliable, high-quality data to support product performance, regulatory compliance, and market readiness.
Total Fat Analysis by CEM ORACLE (NMR Technology)
We determine total fat using the CEM ORACLE fat analyzer, an advanced system based on nuclear magnetic resonance (NMR) technology. This method provides rapid, accurate fat quantification without the need for solvent extraction.
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How the ORACLE System Works A homogenized sample is weighed and introduced into the analyzer. The instrument applies a magnetic field and radiofrequency pulses. Hydrogen nuclei within the sample respond to the magnetic field, and signals from lipid-associated hydrogen are distinguished from non-fat components. Total fat content is calculated directly from the lipid signal. Because fats contain a higher concentration of hydrogen than proteins or carbohydrates, this approach allows for highly selective and precise measurement.
Key Advantages Rapid analysis (results in minutes) · No solvent extraction required · Minimal sample preparation · High accuracy across diverse matrices · Excellent precision and reproducibility · Non-destructive testing approach
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Applications Nutrition Facts panel development · Product formulation and validation · Routine quality control · Raw material and finished product testing · Label claim verification
Fatty Acid Profile by FAMEs Analysis (GC-FID)
We perform fatty acid profiling by converting lipids into fatty acid methyl esters (FAMEs) followed by separation and quantification using gas chromatography with flame ionization detection (GC-FID). This method provides detailed characterization of fatty acid composition, supporting both nutritional labeling and advanced product development.
Fatty Acids We Commonly Analyze DHA (docosahexaenoic acid) · EPA (eicosapentaenoic acid) · CLA (conjugated linoleic acid) · Alpha-linolenic acid (ALA) · Linoleic acid · Oleic acid · Palmitic acid · Stearic acid · Arachidonic acid · Additional saturated, monounsaturated, and polyunsaturated fatty acids
Applications Omega-3 and omega-6 verification · Fish oil and algal oil analysis · Specialty oil characterization · Ingredient identity confirmation · Nutritional labeling support · Product formulation and R&D
Cholesterol Analysis by Derivatization (GC-FID)
We offer cholesterol quantification using derivatization followed by GC-FID, enabling accurate measurement in complex food and supplement matrices.
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Applications Animal-derived ingredients · Dairy and egg-based products · Meat products · Nutritional supplements · Label claim verification
Peroxide Value (Oxidation Testing)
Peroxide value testing measures primary lipid oxidation, providing insight into product freshness, stability, and shelf life. Monitoring peroxide value helps detect early-stage rancidity and supports quality assurance for oxidation-sensitive products.
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Applications Edible oils and oil blends · Fish oil and omega-3 supplements · Softgels and encapsulated oils · Stability and shelf-life studies · Raw material quality control
Applications for Fat & Lipid Testing
Nutrition labeling (NLEA compliance) · Product specification verification · Ingredient qualification · Research and development · Stability and shelf-life studies · Supplier verification · Claims substantiation · Routine quality control
Need help with your Nutrition Facts label?
Understanding how to translate your fat analysis and fatty acid profiles into compliant packaging can be complex. For comprehensive information on formatting, declarations, and regulatory requirements, we recommend reviewing the FDA's Guidance for Industry: Food Labeling Guide